ABSTRACT
Objective For the purpose of finding new bioactive compounds from natural resources, the phytochemical investigation on the fruiting bodies of Amauroderma rude was carried out. Methods The chemical constituents from A. rude were isolated by various technologies, such as silica gel, Sephadex LH-20, MCI-gel resin, and high performance liquid chromatography. The isolated compounds were elucidated by spectroscopic methods, including extensive 1D, 2D NMR, and ESI-MS techniques. Results Three compounds (1-3) including a new norlignan, were isolated from this mushroom. Their structures were identified as 4-ethyoyl-E-3-(3,4-dihydroxybenzylidene)-5-(3,4-dihydroxyphenyl)furan-2-one (1), esculetin (2), and caffeic acid (3). Conclusion Compound 1 is a new compound isolated from this fungus, which is identified as amauroderin A.
ABSTRACT
Objective: Study on the chemical constituents in the ethyl acetate phase extracted from the fruiting bodies of Amauroderma rude. Methods: Silica gel column chromatography, Sephadex LH-20 gel and liquid chromatography were used to separate and purify the chemical constituents in fruiting bodies of Amauroderma rude. The compounds were identified according to their physical and chemical properties and spectroscopic data. Results: Three compounds including one new lignan and two known sterols were isolated from the ethyl acetate phase of this fungus. Their structures were identified as 3,4-dihydroxyphenacyl acetyl ketone (1), (22E, 24R)-3β,5α-dihydrox-yerogosta-7,22-diene-6-one (2), and (22E,24R)-ergosta-6,9,22-trien-3β,5α,8α-triol (3). Conclusion: Compound 1 was a new lignan and named amaurolignan B.
ABSTRACT
Objective: To study the chemical constituents from the fruiting bodies in Amauroderma rude of family Ganodermataceae. Methods: The constituents were separated by column chromatography and their structures were elucidated by spectral data analyses. The cytotoxicities of compounds were evaluated using MTT methods. Results: Twelve compounds were isolated and identified from the fruiting bodies of A. rude and were identified as diptoindonesin D (1), 6-deoxyjacareubin (2), jacareubin (3), 1H-indole-3-carboxylic acid (4), methyl 3,4-dihydroxybenzoate (5), 3,4-dihydroxyphenylethanol (6), 3β-hydroxy-7, 22E-dien-ergosta (7), 3β, 7α-dihydroxy-8,22E-5α, 6α-epoxyergosta (8), 3β-hydroxy-7α-methoxy-8 (14), 22E-dien-5α, 6α-epoxyergosta (9), ergosterol 5α, 8α-peroxide (10), 3β-5β-8β-trihydroxy-6, 22E-ergosta (11), and 3β, 5α-6β-trihydroxy-7, 22E-dien-ergosta (12). Compounds 2 and 3 exhibited the cytotoxic activities against HL-60, SMMC-7721, A-549, MCF-7, and SW-480 cell lines and compound 9 showed the cytotoxic activities against HL-60, MCF-7, and SW-480 cell lines. Conclusion: All the compounds are obtained from this fungus for the first time. Compounds 2,3, and 9 show the definite cytotoxicities against five cell lines.